WEBVTT

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Obtain a frog leg.

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The frog is cut at the hip bone to separate it

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this allows you to use both legs.

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First, isolate the gastrocnemius.

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Be careful with both of these as you dissect the frog.

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Use a wooden probe,

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toothpicks, or glass probe.

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Move the probe directly underneath the muscle and separate it from the bone.

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Move the probe along the bone towards the foot.

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Remove as much of the Achilles tendon as possible

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while being very gentle with the muscle.

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If you have removed too much of the Achilles tendon,

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you can trim it back later.

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Cut the tendon.

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You can put a hook on it at this point,

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or after the rest of the dissection.

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Don't use metal with nerves,

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because metal can damage the nerve.

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Separate the muscle from the bone so the bone is easier to cut.

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You may want to remove a little bit of the muscle,

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so the leg will be easier to work with

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when the remaining bone is cut.

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Find the sciatic nerve.

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It's usually on the back part of the leg.

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Do a blunt dissection through the hamstrings.

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Look for many vessels

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because they surround the nerve.

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The sciatic nerve is the silver string.

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Keep 2.5 cm of material for the muscle holder.

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Expose the bone

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and the area where the nerve comes through.

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Carefully cut the muscle to expose the bone and nerve.

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The dissected leg can have all of the vessels attached.

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You don't want to sever the sciatic nerve at the knee joint.

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Place the sciatic nerve on the gastrocnemius.

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Cut some of the tissue away from the bone and nerve

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so you can identify where you want to cut the femur and the tibio-fibula.

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Find the knee joint, it looks like a big ball.

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The sciatic nerve should innervate the knee joint, and the muscle at the joint.

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Cut the tibio-fibula to keep the bulk of the ball,

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and cut the femur.

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At this point, the frog leg is ready for the muscle holder.

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You can do some extra trimming if you need to.

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Make sure the nerve is isolated.

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Place the dissected leg into the muscle holder.

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Make sure that the nerve is touching the bottom electrodes.

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You can easily switch stimulation of the muscle or the nerve,

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by moving your stimulating electrode cables to different connectors.

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The two inner connectors are for the nerve stimulation.

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The two outer connectors are for the muscle stimulation.