﻿WEBVTT

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Obtain and wear gloves throughout the procedure.

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Connect one end of the rubber tube to the syringe
and the other end to the glass pipette.

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Slowly pull the plunger to collect 0.5 parts of semen solution.
The solution is colored blue for demonstration purposes.

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Wipe off any excess solution from the outside of the pipette.

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Fill the pipette with dilution solution up to the 11 part mark.
The dilution solution is colored pink for demonstration purposes.

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To mix the solution, hold the pipette horizontally and use your fingers to slowly roll the pipette.

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Roll the pipette until the two solutions are mixed thoroughly.

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Discard the first few drops of mixed solution onto a paper towel.

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Place the Improved Neubauer Chamber on a flat surface and place a coverslip on top.

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Place the end of the pipette next to the coverslip above the central platform.

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Slowly release the plunger to allow a droplet to form.

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The droplet will enter the chamber through capillary action
when it comes into contact with the coverslip.

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Only one droplet should fill the chamber.
Take care not to add extra solution.